Clostridium botulinum Type F: Isolation from Venison Jerky

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Clostridium botulinum type F: isolation from venison jerky.

A Clostridium botulinum type F was isolated from the venison jerky responsible for the only type F botulism outbreak reported in the United States. The isolate differed from the prototype Langeland type F strain in being nonproteolytic.

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Purification and properties of Clostridium botulinum type F toxin.

Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...

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Molecular construction of Clostridium botulinum type F progenitor toxin.

Molecular dissociation of purified type F progenitor toxin with an S20,W of 10.3 and a molecular weight of 235,000 into two components, toxic and atoxic, was demonstrated by ultracentrifugation, gel filtration, and diethylaminoethyl-Sephadex chromatography at pH 7.5. The ultracentrifugal analysis indicated that type F progenitor toxin dissociates into components of the same molecular size of 5....

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1972

ISSN: 0003-6919

DOI: 10.1128/am.24.2.165-167.1972